Table 1.
Plasmids used in this study
| Plasmid | EpaA variant | Relevant genotype | Source or reference |
|---|---|---|---|
| pET-28(a)+ | PT7 6×His lacI KanR | Merck, Germany | |
| BHUM1829 | Epa1A | EPA1(31-271) in pET-28(a)+ | 15 |
| BHUM1784 | Epa2A | EPA2(32-262) in pET-28(a)+ | 15 |
| BHUM1788 | Epa3A | EPA3(28-264) in pET-28(a)+ | 15 |
| BHUM1790 | Epa6A | EPA6(26-271) in pET-28(a)+ | 15 |
| BHUM1886 | Epa9A | EPA9(39-305) in pET-28(a)+ | 15 |
| BHUM2495 | Epa10A | EPA10(39-306) in pET-28(a)+ | 15 |
| BHUM1804 | Epa1ACBL2Epa2 | EPA1(31-271; E227D; Y228N; D229N) in pET-28(a)+ | This study |
| BHUM1805 | Epa1ACBL2Epa3 | EPA1(31-271; R226I; E227G; Y228K) in pET-28(a)+ | This study |
| BHUM1806 | Epa1ACBL2Epa6 | EPA1(31-271; E227D; Y228N) in pET-28(a)+ | This study |
| BHUM2498 | Epa1ACBL2Epa9 | EPA1(31-271; E227D; D229H) in pET-28(a)+ | This study |
| BHUM3162 | Epa1AL1Epa9 | EPA1(31-271; R71-P77::L73-Q108(EPA9)) in pET-28(a)+ | This study |
| BHUM2504 | Epa3ACBL2Epa1 | EPA3(28-264; I219R; G220E; K221Y) in pET-28(a)+ | This study |
| BHUM2506 | Epa3ACBL2Epa2 | EPA3(28-264; I219R; G220D; K221N, D222N) in pET-28(a)+ | This study |
| BHUM3163 | Epa6AL1Epa9 | EPA6(26-271; L71-P77::L73-Q108(EPA9)) in pET-28(a)+ | This study |
| BHUM2510 | Epa9ACBL2Epa1 | EPA9(39-305; D258E; H260D) in pET-28(a)+ | This study |
| BHUM3165 | Epa9AL1Epa1 | EPA9(39-305; L73-Q108::R71-P77(EPA1)) in pET-28(a)+ | This study |
| BHUM3166 | Epa9AL1Epa10 | EPA9(39-305; L73-Q108::L73-Q109(EPA10)) in pET-28(a)+ | This study |
| BHUM3164 | Epa10AL1Epa9 | EPA10(39-306; L73-Q109::L73-Q108(EPA9)) in pET-28(a)+ | This study |
| BHUM1760 | PFLO11-FLO11(1-30)-SP-3HA-FLO11(214-1360)-TFLO11 in YCplac33 | 15 | |
| BHUM1983 | Epa1A | EPA1(31-271) in BHUM1760 | 15 |
| BHUM1985 | Epa2A | EPA2(32-262) in BHUM1760 | 15 |
| BHUM2150 | Epa3A | EPA3(28-264) in BHUM1760 | 15 |
| BHUM2020 | Epa9A | EPA9(39-305) in BHUM1760 | 15 |
| BHUM3167 | Epa10A | EPA10(39-306) in BHUM1760 | 15 |
| BHUM2171 | Epa1AC78S | EPA1(31-271; C78S) in BHUM1760 | This study |
| BHUM2172 | Epa1AD165A | EPA1(31-271; D165A) in BHUM1760 | This study |
| BHUM2173 | Epa1AW198A | EPA1(31-271; W198A) in BHUM1760 | This study |
| BHUM2174 | Epa1AW198H | EPA1(31-271; W198H) in BHUM1760 | This study |
| BHUM2175 | Epa1AW198Y | EPA1(31-271; W198Y) in BHUM1760 | This study |
| BHUM1984 | Epa1ACBL2Epa2 | EPA1(31-271; E227D; Y228N; D229N) in BHUM1760 | 12 |
| BHUM2016 | Epa1ACBL2Epa3 | EPA1(31-271; R226I; E227G; Y228K) in BHUM1760 | 12 |
| BHUM1995 | Epa1ACBL2Epa6 | EPA1(31-271; E227D; Y228N) in BHUM1760 | 12 |
| BHUM2160 | Epa1ACBL2Epa9 | EPA1(31-271; E227D; D229H) in BHUM1760 | This study |
| BHUM3162 | Epa1AL1Epa9 | EPA1(31-271; R71-P77::L73-Q108(EPA9)) in BHUM1760 | This study |
| BHUM3185 | Epa1ACBL2+L1Epa9 | EPA1(31-271; E227D; D229H; R71-P77::L73-Q108(EPA9)) in BHUM1760 | This study |
| BHUM2189 | Epa2ACBL2Epa1 | EPA2(32-262; D218E; N219Y; N220D) in BHUM1760 | This study |
| BHUM2190 | Epa2ACBL2Epa3 | EPA2(32-262; R217I; D218G; N219K; N220D) in BHUM1760 | This study |
| BHUM2191 | Epa3ACBL2Epa1 | EPA3(28-264; I219R; G220E; K221Y) in BHUM1760 | This study |
| BHUM2192 | Epa3ACBL2Epa2 | EPA3(28-264; I219R; G220D; K221N, D222N) in BHUM1760 | This study |
| BHUM2018 | Epa6A | EPA6(26-271) in BHUM1760 | 15 |
| BHUM2193 | Epa6ACBL2Epa1 | EPA6(26-271; D227E; N228Y) in BHUM1760 | This study |
| BHUM3163 | Epa6AL1Epa9 | EPA6(26-271; L71-P77::L73::Q108(EPA9)) in BHUM1760 | This study |
| BHUM2194 | Epa9ACBL2Epa1 | EPA9(39-305; D258E; H260D) in BHUM1760 | This study |
| BHUM3165 | Epa9AL1Epa1 | EPA9(39-305; L73-Q108::R71-P77(EPA1)) in BHUM1760 | This study |
| BHUM3166 | Epa9AL1Epa10 | EPA9(39-305; L73-Q108::L73-Q109(EPA10)) in BHUM1760 | This study |
| BHUM3187 | Epa9ACBL2+L1Epa1 | EPA9(39-305; D258E; H260D; L73-Q108::R71-P77(EPA1)) in BHUM1760 | This study |
| BHUM3192 | Epa10ACBL2Epa1 | EPA10(39-306; D259E; H261D) in BHUM1760 | This study |
| BHUM3191 | Epa10AL1Epa1 | EPA10(39-306; L73-Q109::R71-P77(Epa1)) in BHUM1760 | This study |
| BHUM3164 | Epa10AL1Epa9 | EPA10(39-306; L73-Q109::L73-Q108(Epa9)) in BHUM1760 | This study |
| BHUM3193 | Epa10ACBL2+L1Epa1 | EPA10(39-306; D259E; H261D; L73-Q109::R71-P77(Epa1)) in BHUM1760 | This study |