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. 2020 Jul 15;295(35):12512–12524. doi: 10.1074/jbc.RA120.013968

Table 2.

Data collection statistics for Epa complexes obtained in this study

Parameter Epa9A–Galβ1-4Glc Epa9A–Galβ1-3GlcNAc Epa9A–Galβ1-4GlcNAc Epa9ACBL2Epa1–Galβ1-4Glc Epa1ACBL2Epa9–Galβ1-4Glc
PDB code 4CP0 4CP1 4CP2 6Y98 6Y9J
Data collection
    X-ray source BESSY II BL14.1 BESSY II BL14.1 BESSY II BL14.1 ESRF ID23-1 BESSY II BL14.1
    Space group P3121 P3121 P3121 P3121 C2221
    Unit cell (Å) a = b = 66.6, c = 118.0 a = b = 66.6, c = 117.7 a = b = 66.8, c = 116.0 a = b = 65.3, c = 121.9 a = 74.2, b = 104.1, c = 69.1
    Resolution range (Å) 41.23–2.15 (2.27 - 2.15) 41.21–2.50 (2.64–2.50) 40.96–2.58 (2.72–2.58) 40.63–2.8 (2.9–2.8) 34.54–1.1 (1.139–1.1)
    No. of reflections 186,187 52,916 79,850 15,594 212,713
    Rmerge (%) 9.9 (59.2) 13.7 (50.7) 12.3 (57.8) 1.8 (44.0) 2.1 (73.9)
    I/σ(I) 24.9 (4.9) 12.5 (3.4) 15.7 (3.7) 19.5 (1.7) 13.2 (1.0)
    Completeness (%) 99.9 (100) 99.7 (99.9) 99.9 (100) 99.2 (100) 99.1 (92.2)
    Multiplicity 10.9 (11.1) 4.8 (4.9) 8.1 (8.0) 2.0 (2.0) 2.0 (1.9)
Refinement
    Rwork/Rfree (%) 16.3 (19.9)/19.4 (24.9) 17.0 (24.0)/23.1 (38.1) 18.1 (25.0)/24.2 (30.0) 20.6 (35.3)/25.0 (40.7) 14.7 (30.5)/16.7 (30.8)
    No. of atoms 2043 2008 1930 1861 2359
    Avg B factor (Å2) 27.86 21.83 36.07 124.34 19.96
    r.m.s.d.
        Bond length (Å2) 0.009 0.012 0.009 0.004 0.01
        Bond angles (°) 1.263 1.498 1.330 1.02 1.46
    Ramachandran plot
        Favored (%) 95.8 94.9 95.8 94.1 96.5
        Allowed (%) 4.2 5.1 4.2 4.5 3.5
        Outliers (%) 0.0 0.0 0.0 1.4 0.0

aValues for the outer shell are shown in parentheses.