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. 2020 Sep 1;7:150. doi: 10.1038/s41438-020-00372-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Venn diagram showing the number of differentially expressed genes (DEGs) in 4-month-old leaves from the transgenic grapevine line (#25) compared with Thompson Seedless (WT) under control and drought conditions for 20 days. The DEGs in red circles were selected as candidate genes for further analysis. b Using DREME motif analysis, a potential G-box motif sequence was found in the promoter regions of candidate genes. c Venn diagram of putative target genes among the candidate genes compared with the predicted 21 candidate VlbZIP30 target genes. Quantitative real-time (qRT)-PCR analysis of drought-responsive genes (d) and lignin biosynthetic genes (e) in leaves of the VlbZIP30-overexpressing grapevine line (#24, #25, and #27) and WT plants under control and drought conditions. f qRT-PCR analysis of lignin biosynthetic genes in stems of transgenic grapevines and WT plants under control and drought conditions. The first to fourth successive internodes from the top of the plants were sampled. Values are means ± SEs (n = 3). Statistically significant differences are indicated by different lowercase letters according to Fisher’s LSD test (P < 0.05)