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. 2020 Sep 1;7:139. doi: 10.1038/s41438-020-00360-7

Fig. 1. HR fruits accumulated high lignin content in their juice sacs at late developmental stages.

Fig. 1

a The juice sacs of four pummelo cultivars, Kao Pan (KP), Fenghuang (FH), Hirado Buntan (HB), and Huanong Red-fleshed (HR), were collected from mature fruits at 205 DPA. b The juice sacs were separated (left) and stained with phloroglucinol hydrochloride dye (right). The high lignin contents in the HR juice sacs resulted in a dark red color (b, c). d The HR juice sacs were stained dark red and their sections were stained red, indicating a relatively high level of lignin accumulation. The bar applies to all the images in the same panel. e Paraffin sections of KP and HR juice sacs were stained with toluidine blue. The arrowheads point to blue-stained thickened cell walls with a high lignin content. f The lignin content in the juice sacs of four pummelo cultivars was measured at six developmental stages. In HR, the lignin content increased significantly and accumulated much more than that in the other cultivars after 145 DPA. The asterisks indicate significant differences in HR compared with KP, FH, and HB at 175 and 205 DPA, and were analyzed by Duncan’s multiple comparison test (***P < 0.05)