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. 2020 Sep 1;7:139. doi: 10.1038/s41438-020-00360-7

Fig. 5. CgMYB58 bound to and transactivated the promoters of four lignin biosynthetic genes.

Fig. 5

a CgMYB58 increased the activities of the promoters of CgPAL1, CgPAL2, Cg4CL1, and CgC3H according to a dual LUC assay. LUC: luciferase. Pro: promoter; EV: empty vector; the asterisks indicate significant differences between the CgMYB58 effector and empty vectors according to a t-test (***P < 0.05). b CgMYB58 could interact with the promoters of CgPAL1 and CgC3H according to yeast one-hybrid assays. AbA: aureobasidin A; SD/-Leu: SD plate lacking leucine; P: promoter; a pGADT7 empty vector was used as negative control. c CgMYB58 directly bound to the AC elements in the four promoters according to EMSAs. EMSAs: electrophoretic mobility shift assays; FAM: 5’-FAM-labeled oligonucleotide; Pro: probe; Mu: mutant probe