Figure 4.
Anti-dsDNA and nucleohistone reactive fecal IgA antibodies in SNF1 mice. Fecal samples were collected from individual SNF1 males, SNF1 females and B6 females at 4, 8 and 16 weeks of age. (A) Fecal pellets were subjected to ELISA to determine anti-dsDNA and nucleohistone reactivity as detailed in Materials and method. Mean ± SD of ELISA titer per gram feces (16 mice/group) are shown for each time point. (B) Fecal samples from an independent cohort of age-matched seropositive and seronegative mice (between 16 and 24 weeks of age) were also subjected to ELISA for anti-dsDNA and nucleohistone IgA levels. Mean ± SD of ELISA titer per gram feces (10 mice/group) are shown.