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. 2020 Aug 31;10:14293. doi: 10.1038/s41598-020-71340-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Immune-related gene expression profiles. (a) Heat map of 519 differentially expressed genes (DEGs; P < 0.05 and fold change > 2). (b) Gene ontology analysis of the DEGs. (c) Gene Set Validation Analysis to assess the enrichment of gene sets related to immune checkpoints, immune checkpoint ligands, co-stimulatory molecules, co-stimulatory ligands, and chemokines. (d) Gene Set Enrichment Analysis to assess the enrichment of 18-gene T-cell inflamed gene expression profiles. PGC primary gastric cancer, MGC metastatic gastric cancer, NSE normalized enrichment score, GEP gene expression profile.