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. 2020 Sep 1;7:142. doi: 10.1038/s41438-020-00363-4

Fig. 1. Fluorescence and sequencing of genome-integrated GFP sequence in control (untransformed) and cGFP-i or gGFP-i transgenic plants.

Fig. 1

a–g Control untransformed plants. b–h From plant transformed with a vector-expressing GFP under a constitutive promoter and Cas9 under PPC2 promoter, without a gRNA (cGFP). c–i Plants transformed with the same construct of the GFP control, added with gRNA targeting Cas9 to GFP (gGFP). a–c Open flower, d–f fruit, 6 days after pollination, g–i fruit, breaker stage, and j–l DNA sequencing of target GFP site of c–i, respectively. Arrow indicates the Cas9 target site. Sequence shown is bases 3254–3277 of GenBank accession EF090408 of GFP.