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. 2020 Aug 31;11:4368. doi: 10.1038/s41467-020-18184-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a IL-23 receptor expression on unstimulated CD4⁺ cells in healthy controls (n = 8) and SLT patients (n = 7): HC 0.42% (0.34–1.01), SLT 0.79% (0.73–2.60), p = 0.07. Groups are compared with a two-sided Mann–Whitney test. Error bars represent interquartile range around the median. b IL-23 receptor expression on unstimulated CD4⁻ cells in healthy controls (n = 8) and SLT patients (n = 7): HC 3.34% (2.26–6.56), SLT 9.67% (5.64–10.65), p = 0.02. Groups are compared with a two-sided Mann–Whitney test. Error bars represent interquartile range around the median. c Relative lymphocyte NFAT5 expression in healthy controls (n = 13) and SLT patients (n = 11): HC 1.01 AU (0.70–1.95), SLT 1.10 AU (0.76–1.62), p = 0.85. Groups are compared with a two-sided Mann–Whitney test. Error bars represent interquartile range around the median. d Relative lymphocyte SGK1 expression in healthy controls (n = 7) and SLT patients (n = 13): relative intensity HC = 1.07 AU (0.73–1.2), SLT = 1.09 AU (0.80–1.31), p = 0.70. Groups are compared with a two-sided Mann–Whitney test. Error bars represent interquartile range around the median. e Representative western blot of SGK1 expression in HCs and SLT patients. SGK1 expression was determined in SLT patients (n = 13) and healthy controls (n = 7) across three experiments. f Th17 polarisation in HC (n = 27) and SLT patients (n = 25) in standard media, and in SLT patients in media supplemented with 40 mM NaCl: HC + 0 mM NaCl 3.2% (2.5–6.3); SLT + 0 mM NaCl 1.6% (0.8–2.0); SLT + 40 mM NaCl 3.1% (2.4–5.3); p = <0.0001. Groups are compared using a Kruskal–Wallis test with Dunn’s multiple comparison testing (shown with significance bars). Error bars represent interquartile range around the median. g Tc17 polarisation in HC (n = 27) and SLT patients (n = 25) in standard media, and in SLT patients in media supplemented with 40 mM NaCl: HC + 0 mM NaCl 1.5% (0.6–3.0); SLT + 0 mM NaCl 0.6% (0.3–1.1); SLT + 40 mM NaCl 1.6% (0.9–3.1); p = 0.0007; groups are compared using a Kruskal–Wallis test with Dunn’s multiple comparison testing (shown with significance bars). Error bars represent interquartile range around the median. ns not significant (p > 0.05), *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. HC healthy control, SLT salt-losing tubulopathy, AU arbitrary units. Source data are provided as a Source data file.