Table 1.
Sequences and frequencies of high-affinity oligopeptides from plant proteins digested in silico by gastrointestinal enzymes
| Protein names [amino acids length] | Accession number | Sequences of high-affinity oligopeptides (length < 6 and docking score < −100) binding to receptor-binding domain of SARS-CoV-2 spike RBD [Binding score] | Frequency of high-affinity oligopeptide in a protein (F) |
|---|---|---|---|
| Arthrospira platensis | |||
| Phycocyanin alpha chain [162] | ABD64608.1 | TTQM[−103.3],DISY[−123.9],SPSW[−131.2],IEAL[−100.4] | 0.0247 |
| Phycocyanin beta chain [172] | ABD64607.1 | ITSN[−108.4] | 0.0058 |
| Phycobiliprotein lyase [174] | WP_006618130.1 | DIVEF[−125],APDH[−109.5],PDQQR[−136.8],GSAVL[−107.8],TSIVN[−138.4],CSEIR[−115] | 0.0345 |
| Carotenoid biosynthesis protein [306] | WP_014274510.1 | SSEL[−100.4],GSSW[−119.1],DPAM[−100.7], SQAPF[−131.2],PIVL[−119.2] | 0.0163 |
| Photosynthesis system II assembly factor Ycf48r [334] | WP_006616832.1 | IAVAL[−121.1],PDVSY[−135],DIDF[−110],IVGSN[−127.3],TSVSF[−133.4],PGAPN[−124.6], SAAEM[−100.3],VGAIY[−135.4],GGEIR[−112.2],DPEEW[−130.3],SDPIK[−103.5],STSW[−131.9],TSASS[−103.8] | 0.0389 |
| Corn (Zea mays) | |||
| Ribulose bisphosphate carboxylase large chain[476] | P00874.2 | TPEY[−122.1],VTPQL[−124.5],TTVW[−138],VAY[−102.9], PPAY[−132.8],QGPPH[−125.1],GCTIK[−118.6],TGGF[−108.5],AVIDR[−125.4], SGGDH [−101.4],EGER[−107],EITL[−107.5],TQDW[−122.6],TEIF[−111.3],EIIK[−100] | 0.0315 |
| Ribulose bisphosphate carboxylase small chain[170] | P05348.1 | APTVM[−120.7],STASL[−109.6],PVAR[−135.6],QVW[−133],QVDY[−135.8],IPCL[−108.7],QEAIK[−106.7],PDAF[−131.1],VIGF[−111.7],IAY[−106.1],PPGSD[−127.8] | 0.0647 |
| Sorghum (Sorghum vulgare) | |||
| Ribulose bisphosphate carboxylase large chain[476] | A1E9T2.1 | ASVGF[−121.9],TPEY[−122.1],VTPQL[−124.5],TTVW[−138],VAY[−102.9],PPAY[−132.8],QGPPH[−125.1],GCTIK[−118.6],TGGF [−108.5],AVIDR[−125.4],SGGDH [−101.4],EGER[−107],EITL[−107.5],TQDW[−122.6],TEIF [−111.3],EIIK[−100] | 0.0336 |
| Ribulose biophosphate carboxylase small subunit[169] | BAJ40065.1 | APTVM[−120.7],STATL[−104.9],PVAR[−135.6],STTSF[−126.6],QVW[−133],QVDY[−135.8],VPCL[−100.1],IAY[−106.1],PAGSE[−117] | 0.0533 |
| Wheat (Triticum aestivum) | |||
| Alpha/beta-gliadin[286] | P02863.2 | PVPQL[−133],QPQN[−114.9],P SQL[−102],QPF[−113.9],PQPQL[−133.1],PQQPY[−146.1],QQIL[−111.9],IPCM[−110.4],DVVL[−103.9],QQSTY[−134.8],CCQH[−119.8],VVH[−106.8],AIIL[−117.5],QQQK[−105.4],QQQQ[−120.3],QQPL[−107.5],QQY[−111.9],GQGSF[−129.1],PSQQN [−134.8],EEIR[−111.1],IPPY [−133.1],GIF[−100.3] | 0.0769 |
| Glutenin, high molecular weight subunit[660] | P08488.1 | QCER [−111.1],QESSL[−115.9],EACR [−103.4],CCQQL[−131.8],DVSAK [−101.3],GGSF[−117.1],QQGIF[−145.4],QGSY [−123],PTSL[−126.2],PTSL[−126.2],TGQR[−111.7],QQGY[−124.8],PASL[−119],PTSL[−126.2],PTSL[−126.2],PTSL[−126.2],PASL[−119],PTSL[−126.2],DSPY[−118.2],PTVCR[−143] | 0.0303 |
| Glutenin, low molecular weight subunit[307] | P10386.1 | CIPGL[−112.1],QQQPL[−120.1],PQQPL[−126],SQQQP[−115.7],PSIL[−118.7],EAIR[−110],AIIY[−127.1],SIIL[−108.3],AQGTF[−124.7],QIAQL[−121.3],TSIAL[−112.4],CSVN [−107.8] | 0.0391 |
| Ribulose bisphosphate carboxylase large chain[477] | P11383.2 | AGVGF[−116],TPEY[−122.1],TTVW[−138],VAY[−102.9],PPTY[−137.2],QGPPH [−125.1],GCTIK[−118.6],TGGF[−108.5],AVIDR[−125.4],SGGDH[−101.4],EGER[−107],TQDW[−122.6],TEIF[−111.3],EIIR[−109] | 0.0294 |
| Ribulose bisphosphate carboxylase small chain[175] | P26667.1 | APAVM[−116],STAGL[−100.7],PISCR [−154],SSVSN [−117.7],QVW[−133],QVDY[−135.8],VPCL[−100.1],SSPGY[−126.3],PDAY[−131],VIGF[−111.7] | 0.0571 |
| Soybean (Glycine hispida) | |||
| Basic 7S globulin[427] | P13917 | PVQN [−118.6],QVPVL[−116.1],CEQQY[−132.2],QAPF[−122.3],PGCH[−119.4],APISL [−119.4],TTCL[−104.1],PTSK[−124],GAIIF[−132.4],QDIF[−128.7],TITL[−111.6],QGEY[−115.3],QQSVY[−135.9],TQVF[−131.5],AQQL[−104.4],QAQVK [−129.9],SVAPF[−125.7],PSVDL [−125.2],AEITL[−110.6],STSSL[−110.4] | 0.0468 |
| Glycinin G5[516] | P04347 | SSACF[−132.9],ECQL[−105.9],EPDH[−109.9],IETW[−132.5],GAIGF[−118.1],SQQQL [−123.5],QDSH[−106.9],EGDVL [−100.1],VIPL[−102.7],GVPY[−118.6],PDIEH [−125.8],PETM[−109.6],QGQH[−114.7],AQSF[−116.2],QEQED[−110.7],TPSY[−119.7],PPQR[−124.9],GCQTR[−121.1],ISTL[−102.7],SAQY[−115.6],SPDW[−121.1],SVTM[−108.9],CQGN[−102.7],VVPQN [−129.4], AVSSY [−129.4] | 0.0484 |
| Beta-conglycinin beta subunit[439] | P25974 | GTVF[−119.9],SPQL[−103.7],IVQF[−121.1],ADADF[−111.7],AIPVN[−117.4],ETSF[−121.5],EQIR[−109.3],SSSR[−105.4],AIVIL[−137.7],EQQQK[−120.8],EVQR[−106],QIER[−114.7],QVQEL[−130.3],PSIL[−118.7] | 0.0319 |
| Beta-conglycinin alpha subunit[605] | P0DO16 | GIAY [−109.4],QSCN[−107.2],QACH[−111.8],GEIPR[−111],PIPF[−137],PQPR[−124.2],QEEEH [−106.5],EEQEW[−137.7],SPQL[−103.7],ADADY[−106.1],GTAIL[−108.8],AIPVN [−117.4],EASY[−116.5],EQIR[−109.3],SSSR[−105.4],DPIY[−121.4],SIVDM[−114.3],AIVIL[−137.7],AIGIN[−110.1] | 0.0314 |
| Beta-conglycinin alpha’ subunit[621] | P11827 | GVVF[−116.4],GIAY[−109.4],QACH[−111.8],QEEEH[−106.5],QDER[−116.3],ADADY[−106.1, IVIL[−110.4], AIPVN[−117.4],EASY[−116.5],SSSR[−105.4],DPIY[−121.4],SVVDM[−124.1],AIVVL[−126.7],EQQQR[−142.7],PVVVN [−129.6],ATSDL[−102.1],PGSAK[−116.4] | 0.0274 |
| Barley (Hordeum vulgare) | |||
| B hordein precursor[290] | Q40026 | PQQPF[−147.4],PQQPF[−147.4],PQQP[−112.1],PQQPF[−147.4],QQPIL[−125.5],PQGQL[−126.8],QIPY[−128.9],PSIL[−118.7],EAIR[−110],AIVY[−116.2],QIAQL[−121.3],PPDF[−131.5] | 0.0414 |
| D hordein precursor[707] | Q40054 | TTAER[−114.5],QCER[−111.1],QESSL[−115.9],EACR[−103.4],VGQL[−100.8],PVAL[−119.7],SQVVR[−129.1],GGSF[−117.1],QQGGW[−149.7],GTSVK[−102.2],QQSW[−144.4],PGSTF[−132.6],PSATF[−141],QPGQW[−146.5],PSATF[−141],QQGSY[−136.7],GQQL[−103.3],TSPH[−113.1],TQQK[−103.9],PGQGY[−138.8],QPF[−113.9],GQQSN[−112.7],GSPY[−115.6],AQQL[−104.4] | |
| Gamma-hordein-3[289] | P80198.1 | PSGL[−105.7],PQQL[−129],PQQQF[−152.2],PQQM[−119.5],PQQM[−119.5],PQQK[−115.2],QQPL[−107.5],TQQPY[−141.1],QQCTL[−120],ISQQN [−126.6],EQSR[−114.3],AIVM[−104],VQSQL[−119],PIQL[−115.9],VVGSL[−111.3],VIQTL [−125.9],VPPY[−138.5],CSPF[−120.1] | 0.0623 |
| Ribulose bisphosphate carboxylase large chain[479] | P05698.2 | AGVGF[−116],QAGVK[−110.5],TPEY[−122.1],TTVW [−138],VAY[−102.9],PPTY[−137.2],QGPPH[−125.1],GCTIK[−118.6],TGGF[−108.5],AVIDR[−125.4],SGGDH[−101.4],EGER[−107],TQDW [−122.6],TEIF[−111.3],EIIR[−109] | 0.0313 |
| Ribulose bisphosphate carboxylase small chain[174] | Q40004 | APTVM[−120.7],STAGL [−100.7],PVSR[−128.8],QVW[−133],QVDY[−135.8],VPCL[−100.1],ASPGY[−122.1],IIGF[−122.5] | 0.0460 |
| Oat (Avena sativa) | |||
| Avenin[214] | P27919.1 | AQY[−117.3],CQQH[−121],DSCR[−101],VAER[−106.7],CTTM[−102.8],PITW[−143.5],ECCQL[−110.9],PSECR[−135],CDAIW[−130],SIQR[−114.3],SAISP[−106.1],IAGK[−101.7],PVDVL[−115.2],[QEAR[−105.2],TQTSF[−137.2] | 0.0701 |
| 11S globulin precursor[551] | Q38779 | ATTSF[−122.3],QSSR[−124.6],QQQF[−130],QPF[−113.9],DQSQF [−142.2],QVGK[−108.3],STPY[−116.1],DQSF[−106],ADTY[−115],IVQM[−105.1],IQGH[−123.4],VQVVN[−152.4],GQTVF[−146.1],IVPQH[−139.1],EGCQY[−137],IAGK[−101.7],PIDVL[−116.4],QEAR[−105.2],GEEF[−100.4],TQTGF[−130.5] | 0.0363 |
| Ribulose bisphosphate carboxylase large chain[477] | P48684.1 | ASVGF[−121.9],QAGVK[−110.5],TPEY[−122.1],TTVW[−138],VAY[−102.9],PPAY[−132.8],QGPPH[−125.1],GCTIK[−118.6],ITGGF[−125.6],AVIDR[−125.4],SGGDH[−101.4],EGER[−107],TQDW[−122.6],TEIF[−111.3],EIIR[−109] | 0.0314 |
| Ribulose bisphosphate carboxylase small subunit[165] | Q9ZWG4 | STAGL[−100.7],PVGR[−121],QVW[−133],QIDF[−128.3],VPCL[−100.1],PDAY[−131],IIGF[−122.5],PPGCE[−127.7] | 0.0485 |