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. 2020 Aug 31;10:14287. doi: 10.1038/s41598-020-71138-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Bortezomib induces oxidative stress in melanoma cells and inhibits cleavage of Caspase-3. (A) Melanoma cells were treated as indicated for 24 h. The cells were imaged on Zeiss Celldiscoverer 7 using 10 × magnification. The scale bar represents 100 μm. (B) Comparison of the intensity values of the red channel normalized to the cell nuclei. The data were analyzed using ImageJ software. Data in duplicates were expressed as mean ± standard deviation. (C) Proteome profiling of A2058 cells after treated with 20 ng/mL BOZ, 100 μg/mL ALA and their combination for 24 h. On the left panel, the array blots are presented. Spots for each protein are in duplicate. Red squares indicate the proteins quantified on the right panel. (D) Relative protein levels were analyzed using Image Lab 6.0.1 Software. The levels of significance are shown as follows: x: P < 0.05; y: P < 0.01; z: P < 0.001, determined by the One-way ANOVA test followed by Fishers LSD post hoc test.