. 2020 Aug 31;10:14299. doi: 10.1038/s41598-020-71070-2

Table 2.

Information on RT-qPCR primers used in this study.

GenBank accession	Medicago locus	Annotation	Primer sequence (5′–3′)	Gene position	PCR product size (bp)	PCR efficiency	PCR product Tm (°C)
KP006493.1	MTR_1g022445	Vicia faba dihydroflavonol reductase 1 (DFR1)	TCTTGTTTGAGCATATGGAAGTTGAG	Exon 5	111	1.94 ± 0.06	74.12 ± 0.06
KP006493.1	MTR_1g022445	Vicia faba dihydroflavonol reductase 1 (DFR1)	GGACATTGTACTCTGGAAATTTTGCA	Exon 5	111	1.94 ± 0.06	74.12 ± 0.06
XM_003590588.3	MTR_1g072140	Transducin/WD40 repeatprotein	TCCGACTCCCACTACGTCTGC	Exon 1	108	1.93 ± 0.03	84.69 ± 1.01
XM_003590588.3	MTR_1g072140	Transducin/WD40 repeatprotein	GATGTGTGTATGGCCACGGAG	Exon 1	108	1.93 ± 0.03	84.69 ± 1.01
XM_024786476.1	MTR_1g072320	Transcription factor bHLH (TT8)	CGGAGAGACAAATCCGCCAAC	Exon 2	117	1.94 ± 0.02	78.99 ± 0.35
XM_024786476.1	MTR_1g072320	Transcription factor bHLH (TT8)	CCGACACCAGGAGGAAATGAG	Exon 2	117	1.94 ± 0.02	78.99 ± 0.35
XM_003590628.3	MTR_1g072530	Transcription factor bHLH66	CAAGCTTACATACTGGACGACATC	Exon 5–6	110	1.91 ± 0.03	75.90 ± 0.27
XM_003590628.3	MTR_1g072530	Transcription factor bHLH66	ACTAGTGGTATAGCATTTGAGTCA	Exon 5–6	110	1.91 ± 0.03	75.90 ± 0.27
XM_013613397.2	MTR_1g077640	bHLH transcription factor	TAGGAAGTGTGATCAAATATGCAGAC	Exon 7–8	120	1.94 ± 0.03	77.56 ± 0.31
XM_013613397.2	MTR_1g077640	bHLH transcription factor	CTTCAAATGCCCATGTAGCACC	Exon 7–8	120	1.94 ± 0.03	77.56 ± 0.31

GenBank accession, medicago locus, annotation NCBI, primer sequences, gene position and amplicon size. Primer PCR efficiency and PCR product Tm data represent mean values ± sd.