Table 1.
List of antibodies and reagents.
| Anti-CD3ε APC-Cy7 (clone 145-2C11) (1/200) | TONBO | 25-0031-U100 |
| Anti-CD4 BUV395 (clone GK1.5) (1/300) | BD Biosciences | 563,790 |
| Anti-CD4 BV510 (clone RM4-5) (1/200) | BD Biosciences | 563,106 |
| Anti-CD8α BV650 (clone 53-6.7) (1/300) | BD Biosciences | 563,234 |
| Anti-CD19 PE-Cy7 (clone 1D3) (1/400) | BD Biosciences | 552,854 |
| Anti-CD24 BV605 (clone M1/69) (1/1000) | Biolegend | 101,827 |
| Anti-CD24 FITC (clone M1/69) (1/700) | BD Biosciences | 553,261 |
| Anti-CD24 PE/Cy7 (clone M1/69) (1/800) | Biolegend | 101,821 |
| Anti-CD25 APC (clone PC61) (1/800) | Biolegend | 102,012 |
| Anti-CD27 PerCP-eFluor710 (clone LG.GF9) (1/200) | Thermo Fisher Scientific | 46-0271-82 |
| Anti-CD44 PE (clone IM7) (1/300) | Biolegend | 103,008 |
| Anti-CD44 redFluor710 (clone IM7) (1/300) | TONBO | 80-0441-U100 |
| Anti-CD45R/B220 BV711 (clone RA3-6B2) (1/300) | BD Bioscience | 563,892 |
| Anti-CD122 FITC (clone TM-BETA 1) (1/100) | BD Bioscience | 553,361 |
| Anti-CD122 PE (clone TM-b1 [TM-beta1]) (1/100) | Thermo Fisher Scientific | 12-1222-81 |
| Anti-CD183 (CXCR3) PE-Cy7 (clone CXCR3-173) (1/100) | Biolegend | 126,516 |
| Anti-CD186 (CXCR6) BV421 (clone SA051D1) (1/150) | Biolegend | 151,109 |
| Anti-CD196 (CCR6) BV421 (clone 29-2L17) (1/100) | Biolegend | 129,828 |
| Anti-CD279 (PD-1) APC (clone J43) (1/400) | BD Bioscience | 562,671 |
| Anti-IL-25R (IL17RB) Alexa Fluor647 (clone 9B10) (1/200) | Biolegend | 146,304 |
| Anti-IL-25R (IL17RB) PE (clone MUNC33) (1/100) | Thermo Fisher Scientific | 12-7361-80 |
| Anti-γδ T-Cell Receptor BV421 (clone GL3) (1/200) | BD Bioscience | 562,892 |
| Anti-γδ T-Cell Receptor PE (clone GL3) (1/100) | BD Bioscience | 553,178 |
| Anti-γδ T-Cell Receptor PE-CF594 (clone GL3) (1/300) | BD Bioscience | 563,532 |
| Anti-Vγ1.1 (Heilig and Tonegawa’s system: Vγ1) TCR BV421 (clone 2.11)(1/300) | BD Bioscience | 566,308 |
| Anti-Vγ1.1 + Vγ1.2 (Heilig and Tonegawa’s system: Vγ1 + Vγ2) TCR PE (clone 4B2.9) | Biolegend | 142,704 |
| Anti-Vγ2 (Heilig and Tonegawa’s system: Vγ4) TCR BV786 (clone UC3-10A6) (1/300) | BD Bioscience | 742,313 |
| Anti-Vγ3 (Heilig and Tonegawa’s system: Vγ5) TCR BV510 (clone 536) (1/200) | BD Bioscience | 743,239 |
| Anti-Vγ5/Vδ1+ and Vγ6/Vδ1+ (Heilig and Tonegawa’s system: Vγ5Vδ1+ and Vγ6Vδ1+) TCR rat IgM antibody (clone 17D1) | kindly provided by Dr. Robert Tigelaar | |
| Anti- Vγ7 TCR Biotinylated (clone F2.67) (1/600) | kindly provided by Dr. Pablo Pereira | |
| Anti-TCRβ chain (clone H57-597) (1/200) | BD Bioscience | 560,656 |
| Anti-Vδ 6.3/2 TCR BV711 (clone 8F4H7B7) (1/100) | BD Bioscience | 744,476 |
| Purified CD16/32 (clone 93) (1/200) | Biolegend | 101,302 |
| Anti-EOMES eFluor 450 (clone Dan11mag) (1/100) | Thermo Fisher Scientific | 48-4875-82 |
| Anti-IFN-γ PE-CF594 (clone XMG1.2) (1/200) | BD Bioscience | 562,303 |
| Anti-IL-4-Alexa647 (clone 11B11) (1/100) | Biolegend | 504,110 |
| Anti-IL-4 BV421 (clone 11B11) (1/50) | Biolegend | 504,119 |
| Anti-Ki-67 FITC (clone SolA15) (1/600) | Thermo Fisher Scientific | 11-5698-82 |
| Anti-IL-17A BV650 (clone TC11-18H10) (1/200) | BD Bioscience | 564,170 |
| Anti-PLZF Alexa Fluor647 (clone R17-809) (1/200) | BD Bioscience | 563,490 |
| Anti-PLZF PE-CF594 (clone R17-809) (1/400) | BD Bioscience | 565,738 |
| Anti-RORγt PerCP-Cy5.5 (clone Q31-378) (1/200) | BD Bioscience | 562,683 |
| Anti-RORγt PE-CF594 (clone Q31-378) (1/300) | BD Bioscience | 562,684 |
| Anti-T-bet PE-Cy7 (clone eBio4B10) (1/200) | Thermo Fisher Scientific | 25-5825-82 |
| Anti-rat IgM FITC (clone MRM-47) (1/300) | Biolegend | 408,905 |
| Streptavidin APC-Cy7 (1/400) | BD Bioscience | 554,063 |
| Streptavidin PE | BD Bioscience | 554,061 |
| Streptavidin-R-Phycoerythrin | ProZyme | PJRS25 |
| Anti-PE MicroBeads Ultrapure | Miltenyi Biotec | 130-105-639 |
| Anti-Human CD2 FITC (clone RPA-2.10) (1/50) | BD Bioscience | 555,326 |
| Anti-CD3 BUV395 (clone UCHT1) (1/300) | BD Bioscience | 563,546 |
| Anti-CD3 Alexa Fluor594 (clone UCHT1) (1/300) | Biolegend | 300,446 |
| Anti-CD4 APC/Cy7 (clone OKT4) (1/300) | Biolegend | 317,418 |
| Anti-CD8α Alexa Fluor700 (clone RPA-T8) (1/300) | Biolegend | 301,028 |
| Anti-CD19 V500 (clone HIB19) (1/300) | BD Bioscience | 561,121 |
| Anti-CD45RA BV650 (clone HI100) (1/300) | BD Bioscience | 563,963 |
| Anti-CD45RO BV711 (clone UCHL1) (1/300) | BD Bioscience | 563,722 |
| Anti-CD161 Alexa Fluor488 (clone HP-3G10) (1/25) | Biolegend | 339,924 |
| Anti-TCR Vα7.2 BV785 (clone 3C10) (1/25) | Biolegend | 351,722 |
| Anti-TCR γ/δ PerCP/Cy5.5 (clone B1) (1/25) | Biolegend | 331,224 |
| Anti-TCR Vγ9 BV421 (clone B3) (1/50) | BD Bioscience | 744,034 |
| Anti-TCR δ (TCR Vδ1-Jδ2) FITC (clone TS-1) (1/50) | Thermo Fisher Scientific | TCR2055 |
| Anti-TCR Vδ2 FITC (clone B6) (1/50) | Biolegend | 331,406 |
| Anti-IFN-γ BUV395 (clone B27) (1/25) | BD Bioscience | 563,563 |
| Anti-IL-4 BV605 (clone MP4-25D2) (1/25) | Biolegend | 500,827 |
| Anti-IL-17A APC (clone eBio64DEC17) (1/25) | Thermo Fisher Scientific | 17-7179-42 |
| Anti-T-bet PE-Cy7 (clone eBio4B10) (1:100) | Thermo Fisher Scientific | 25-5825-82 |
| Anti-PLZF Alexa Fluor647 (clone R17-809) (1/100) | BD Bioscience | 563,490 |
| Anti-RORγt PE (clone Q21-559) (1/20) | BD Bioscience | 563,081 |