Table 1.
Extracted cfDNA amount and detected genetic alterations.
| ID | Tumor/control | Clinical diagnosis | CSF volume for ctDNA extraction (ml) | cfDNA concentration (ng/μL) | Input cfDNA (ng) | Genetic alterations | AF% |
|---|---|---|---|---|---|---|---|
| 06 | Control | CMV | 1.28 | 17.5 | 20.00 | ND | |
| 13 | Control | NPH | 2.88 | 0.876 | 9.11 | ND | |
| 08 | Tumor | Germinoma | 2.88 | 0.684 | 7.11 | ND | |
| 20 | Tumor | Recurrent germinoma | 1.78 | 0.556 | 5.78 | MAP2K1 p.F129L | 1.6 |
| 21 | Tumor | Mature teratoma | 2.28 | 45.2 | 20.00 | ND | |
| 02 | Tumor | Germinoma | 2.88 | 2.52 | 20.00 | ND | |
| 04 | Tumor | Yolk-sac tumor | 1.38 | 0.302 | 3.14 | NRAS p.Q61R | 34.6 |
| 05 | Tumor | Germinoma | 1.38 | 0.266 | 2.77 | ND | |
| 10 | Tumor | Germinoma | 1.38 | 0.466 | 4.85 | KIT p.D820G | 18.7 |
| 12 | Tumor | GERMINOMA | 0.88 | 0.264 | 2.75 | ND | |
| 15 | Tumor | Germinoma | 1.38 | 0.536 | 5.57 | NRAS p.G12S | 40.3 |
| KIT p.N822Y | 32.3 | ||||||
| 16 | Tumor | Germinoma | 1.38 | 0.216 | 2.25 | ND | |
| 17 | Tumor | Immature teratoma | 1.38 | 0.342 | 3.56 | ND | |
| 19 | Tumor | Germinoma | 0.38 | 0.518 | 5.39 | ND |
CMV cytomegalovirus infection, NPH normal pressure hydrocephalus, AF allele frequency, ND not detected.