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. 2020 Aug 31;10:14317. doi: 10.1038/s41598-020-71330-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of LIPUS on the some signaling molecules in the kidneys of an AKI mouse model. The LIPUS treatment was performed before IRI procedure and after IRI until the day of euthanization. The changes in immunohistochemistry and real-time PCR were determined. (A) The stainings for CHOP, cleaved caspase-3, Cox-2, and catalase in the kidneys were shown. The representative images of at least three independent experiments were shown. Scale bar = 100 μm. (B) The mRNA expression for CHOP in the kidneys was shown. Two reference genes of β-actin and Gapdh were used. Data are presented as mean ± SEM (n = 4–6). *p < 0.05, versus Sham; #p < 0.05, versus IRI alone.