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. 2020 Aug 31;10:14325. doi: 10.1038/s41598-020-71297-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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ENA-like proteins in Aspergillus nidulans. (A) Proposed two-dimensional model of EnaA and EnaB describing the predicted membrane topology and principal conserved functional domains among P-type ATPases. (B) Maximum-likelihood tree of Na⁺, K⁺, H⁺ ATPases from fungi and yeast. EnaA and EnaB clustered in the same group of ATPases while EnaC clearly differentiated and belong to a separate clade. MEGA7 software was used to generate the tree⁵⁸ and it was edited with iTOL⁵⁹. Blue circles in each clade represent bootstrap values. Species; Asp_clav: Aspergillus clavatus; Asp_fisc: Aspergillus fischeri; Asp_fumi: Aspergillus fumigatus; Asp_nidu: Aspergillus nidulans; Asp_nige: Aspergillus niger; Asp_oryz: Aspergillus oryzae; Deb_hans: Debariomyces hansenii; Fus_oxys: Fusarium oxysporum; Hor_wern: Hortaea werneckii; Kom_pahf: Komagataella phafii; Neu_cras: Neurospora crassa; Pen_chry_ Penicillium chrysogenum; Pyr_oryz_ Pyricularia oryzae; Sac_cere: Saccharomyces cerevisiae; Sch_occi: Schwanniomyces occidentalis; Tol_delb: Torulaspora delbrueckii; Unc_rees: Uncinocarpus reesii; Ust_mayd: Ustilago Maydis; Zyg_roux: Zygosaccharomyces rouxii. Cop_cine: Coprinopsis cinerea; Cry_neof: Cryptococcus neoformans; Puc_gram: Puccinia graminis; Tub_mela: Tuber melanosporum; Yar_lipo: Yarrowia lipolytica; Sch_pomb: Schizosaccharomyces pombe; Neo_irre: Neolecta irregularis; Zym_trit: Zymoseptoria tritici.