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. 2020 Aug 31;10:14325. doi: 10.1038/s41598-020-71297-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Functional analysis of null ena mutants. (A) Spores of single-, double- and triple-null strains were point-inoculated and images of colonies were taken after 48 h of incubation at 37 °C in solid AMM (row 1), AMM supplemented with 1 M NaCl (Na⁺; row 2) or 0.3 M LiCl (Li⁺; row 3), AMM adjusted to pH 8 with 50 mM Tris–HCl pH8 (alkaline pH; row 4), and AMM that combined the Tris–HCl buffer with either 1 M NaCl (pH 8 + Na⁺; row 5) or 0.3 M LiCl (pH 8 + Li⁺; row 6). (B) Biomass production was determined by measuring the dry weight of cells grown in liquid AMM for 24 h at 37 °C with and without elevated concentration of cations (1.0 M NaCl and 0.3 M LiCl) or alkaline pH. Data is presented as percentages and normalized by designating the growth of each strain in standard AMM (no stress agents) as 100%. Graphs show the means of three replicates per strain and condition, and error bars indicate standard deviation. Asterisks indicate a significant variation in biomass production (p < 0.05) as compared to the reference wild-type strain. N = 3.