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. 2020 Aug 31;10:14325. doi: 10.1038/s41598-020-71297-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression analysis of ena genes. Northern-blot analyses showing transcript levels of enaA, enaB, enaC and msnA in samples grown in AMM for 18 h (lane C) and subsequently cultivated with 1 M NaCl (Na⁺), 0.3 M LiCl (Li⁺), at pH 8 or in AMM that combined pH 8 with either 1.0 M NaCl (pH8 + Na⁺) or 0.3 M LiCl (pH8 + Li⁺) for additional 10, 30 or 60 min. (A) and (B) show expression levels in a wild-type strain and their quantification, while (C) and (D) show the results corresponding to null enaA and null enaB backgrounds, respectively. Ribosomal RNA (rRNA) stained with methylene blue was used as a loading control. The graphs in (B) show the ratios between average pixel intensity for each hybridization band and the corresponding loading control band. Average pixel intensity for each band was quantified using Image J software (version 4.0; Fujifilm, Valhalla, NY).