Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 31;10:14325. doi: 10.1038/s41598-020-71297-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Constitutive expression of enaA under the control of the gpdA^mini promoter. (A) Expression, in a wild-type background and under inducing (+) or non-inducing (−) conditions, of enaA driven by the native or the gpdA^mini promoters. Strains that integrated one or two copies of the gpdA^mini::enaA plasmid were analyzed. Ribosomal RNA is shown as a loading control. (B) Same experiment as in (A) but carried out with samples corresponding to the palA1 mutant background. (C) Phenotypes of wild-type (left block of images) or palA1 (right) strains bearing zero (–), one (1 ×) or two (2 ×) copies of the gpdA^mini::enaA plasmid after 48 h of culture at 37 °C on AMM (row 1), AMM with LiCl (0.3 M; row 2), KCl (1 M; row 3), NaCl (1 M; row 4) or neomycin (2 mg/mL; row 6), and AMM adjusted to pH 8 (row 5).