Figure 2. Non‐AC40 binding IN1 mutants do not affect Ty1 integration frequency.

1. Localization of GFP₂‐IN1 bNLS variants in yeast cells by direct fluorescence microscopy. Nup49‐mCherry signal was used to visualize the location of the nuclear envelope. Corresponding DIC images are shown. Scale bar is 5 μm.
2. Retrotransposition frequency (log scale) of pGAL1‐Ty1his3AI bearing the indicated substitutions of conserved residues in a spt3‐101 rad52∆ strain. IN1 catalytic core domain mutant D₁₅₄A is defective for integration and NLS mutant (KKR_628–630GGT) for nuclear import. Values are mean ± SD, n = 4 experiments, each performed with four independent colonies. ***P ≤ 0.001, otherwise not significant (Student t‐test).

Source data are available online for this figure.