Table 2.
Sample No. | P2O74− | Pi | 5′-UMP | 2′- Or 3′-UMP | Cyc. UMP | Dimer (U-P-U) | Net Org. PO4 |
---|---|---|---|---|---|---|---|
1 | 67 | 1 | 10.3 | 5.7 | 11 | 5 | 32 |
2 | 90 | 1 | 2.48 | 0.52 | 5 | 1 | 9 |
3 | 92.7 | 0.5 | 2.2 | 0.30 | 3.5 | 0.8 | 6.8 |
4 | 93.8 | 0.2 | 1 | 0.2 | 4.4 | 0.4 | 6 |
5 | 27 | 64 | 7.5 | 1 | 0.5 | ND | 9 |
6 | 66 | 1.5 | 10.8 | 4.2 | 14 | 3.5 | 32.5 |
7 | 91.2 | 1 | 1 | ND | 6 | 0.8 | 7.8 |
8 | 91.3 | 0.3 | 2.72 | 1.28 | 3.4 | 1 | 8.4 |
9 | 89.5 | 0.3 | 1.5 | 0.5 | 7 | 1.2 | 10.2 |
10 | 87.7 | 6.7 | 3.2 | 1.8 | 0.6 | ND | 5.6 |
1 The yields of the phosphorylated products as well as other inorganic phosphates were calculated on the basis of the total phosphorus dissolved and by the peak integration method, coupled to semiquantitative concentration estimation using signal to noise ratios, as previously reported [12,13], where Pi stands for orthophosphate; UMP stands for uridine-monophosphates; cyc. UMP means cyclic uridine monophosphates; and the dimer U-P-U represents uridine-phosphate-uridine, respectively; and ND means not detected. Please see Table 1 for the details relevant with the experimental conditions.