TABLE 5.
Oligonucleotide sequences, plasmids, and E. coli GS1873 strains for the construction of recombinant viruses
| Recombinant virus | Oligonucleotide sequence (5′–3′) | Plasmid DNA template (reference) | E. coli GS1873 straina (source or reference) |
|---|---|---|---|
| YK785 (HSV-2 ΔBAC) | TAGTGAACCGTCAGATCCGCTAGCGCTACCGGTCGCCACCATGCCCAAGAAGAAGAGGAAGGTGTCCA | pcDNA-CREin-KanS (50) | GS1783/pYEbac861 (20) |
| GTAAAACCTCTACAAATGTGGTATGGCTGATTATGATCAGCTAATCGCCATCTTCCAGCA | |||
| YK786 (HSV-2 UL7-SS/AA) | GGTGTATTGGTGGCTTTCGGGGAGCCCCAAACGACGGACCGCGGCGCTTTTCTATCGGTTTTGCAGGATGACGACGATAAGTAGGG | pEP-KanS (48) | GS1783/pYEbac861Cre (this study) |
| ACGTTTATTTTCCGGAGTTAGCAAAACCGATAGAAAAGCGCCGCGGTCCGTCGTTTGGGGCTCCCAACCAATTAACCAATTCTGATTAG | |||
| YK787 (HSV-2 ΔUL7) | GTCGAACGCAGGGATGGCCGACCCCACGCCCGCCGATGAGCTTTCGGGGAGCCCCAAACGAGGATGACGACGATAAGTAGGG | pEP-KanS (48) | Containing the YK786 genome (this study) |
| AGAAAAGCGACGAGGTCCGTCGTTTGGGGCTCCCCGAAAGCTCATCGGCGGGCGTGGGGTCAACCAATTAACCAATTCTGATTAG | |||
| YK789 (HSV-2 Us3-K220M) | TGATAGCAGCCACCCGAACTACCCTCATCGGGTAATCGTCATGGCGGGGTGGTACGCCAGAGGATGACGACGATAAGTAGGG | pEP-KanS (48) | GS1783/pYEbac861Cre (this study) |
| GCCGCGCCTCGTGGCTCGTGCTGGCGTACCACCCCGCCATGACGATTACCCGATGAGGGTCAACCAATTAACCAATTCTGATTAG | |||
| YK790 (HSV-2 Us3-KM-repair) | TGATAGCAGCCACCCGAACTACCCTCATCGGGTAATCGTCAAGGCGGGGTGGTACGCCAGAGGATGACGACGATAAGTAGGG | pEP-KanS (48) | Containing the YK789 genome (this study) |
| GCCGCGCCTCGTGGCTCGTGCTGGCGTACCACCCCGCCTTGACGATTACCCGATGAGGGTCAACCAATTAACCAATCTGATTAG | |||
| YK791 (HSV-1 UL7-Q287R) | CCTCTGGTCTATTGGTGGCTTTCGGAGACCCCAAAACGACGGACGTCGTCGCTGTTTTATCAGGATGACGACGATAAGTAGGG | pEP-KanS (48) | GS1783/pYEbac8102Cre (50) |
| TCCTAAAATTCAACAAAACTGATAAAACAGCGACGACGTCCGTCGTTTTGGGGTCTCCGAACAACCAATTAACCAATTCTGATTAG | |||
| YK792 (HSV-1 UL7-QR-repair) | CCTCTGGTCTATTGGTGGCTTTCGGAGACCCCAAAACGACAGACGTCGTCGCTGTTTTATCAGGATGACGACGATAAGTAGGG | pEP-KanS (48) | Containing the YK791 genome (this study) |
| TCCTAAAATTCAACAAAACTGATAAAACAGCGACGACGTCTGTCGTTTTGGGGTCTCCGAACAACCAATTAACCAATTCTGATTAG | |||
| YK793 (HSV-1 UL7-QSS/RAA) | CCTCTGGTCTATTGGTGGCTTTCGGAGACCCCAAAACGACGGACGGCGGCGCTGTTTTATCAGTTTTGTAGGATGACGACGATAAGTAGGG | pEP-KanS (48) | Containing the YK791 genome (this study) |
| GGTTTATTTCCTAAAATTCAACAAAACTGATAAAACAGCGCCGCCGTCCGTCGTTTTGGGGTCTCCGAACAACCAATTAACCAATTCTGATTAG | |||
| YK794 (HSV-1 UL7-Q287R/Us3-K220M) | GACAGCAGCCACCCAGATTACCCCCAACGGGTAATCGTGATGGCGGGGTGGTACACGAGCACAGGATGACGACGATAAGTAGGG | pEP-KanS (48) | Containing the YK791 genome (this study) |
| CAGTCGCGCCTCGTGGCTCGTGCTCGTGTACCACCCCGCCATCACGATTACCCGTTGGGGCAACCAATTAACCAATTCTGATTAG |
a
Containing HSV-BAC.