TABLE 7.
Summary of constructed plasmids and synthesized plasmids
| Constructed plasmid | Synthesized DNA sequencea |
|---|---|
| pUC57-ChHV-Us3o | AAGCTTGCATGTAGGAAGTTCTGTCGAGTGTATGGAAGACCCGATAAAAGGCAGGAGGCCAGCGTCCCACCCGAGACCAAAACCGCCCCAGTCTTCCCTAGCGGCACCTTTTACACACCTGCAGAGGATGCATATCTGGCATCTGGCTCTCCTGAGACAATCCACCCAAGCCGGCCCCCTTCCCCTGGAGAGGCAGCACGGCTGAGACAGCTGCAGGAGATCCTGGCCCAGATGCACTCCGATGAGGACTACCCTATCGTGGATGCAGCAGGAGCAGAGGAGGGCGGCGAGGCAGACGATGACGCCCCAGATGACGTGGCCTACCCCGATGACTATGCCGAGGGCAGGTTCCTGTCTATGGTGAGCACAGCCCCTCTGCCAGGCGACGGCGGACACCTGCCCGTGCCATGCAGAGCAGCACACTCTGATGTGAGAACCTGTGACTCCACAAAAGTGGGCGCCACCGGCTTTACACCAGAGGAGCTGGATACCATGGACCGGGAGGCCCTGAGAGCCATCTCTAGGGGCTGCAAGCCACCCAGCACACTGGCCAAGCTGGTGACAGGCCTGGGCTTCACAATTCACGGCGCCCTGATCCCTGGATCTGAGGGCTGCGTGTTCGATAGCTCCCACCCTGACTACCCACAGAGAGTGATCGTGAAGGCCGGCTGGTATGCCAGCACCTCCCACGAGGCCAGACTGCTGCGGAGACTGGATCACCCAGCCATCCTGCCTCTGCTGGACCTGCACGTGGTGAGCGGAGTGACCTGCCTGGTGCTGCCCAAGTACCAGTCCGATCTGTACACATATCTGTCTAAGAGGCCAAGCCCCCTGGGACACCTGCAGATTGCAGCCGTGTCCAGACAGCTGCTGTCTGCCATCGATTATGTGCACAGGGAGGGCATCATCCACCGCGACATCAAGACCGAGAACATCTTCATCAATACACCTGAGAACATCTGTCTGGGCGATTTTGGAGCAGCATGCTTTGTGCGGGGATGTAGATCTAGCCCATTTCAGTACGGCATCGCCGGCACCATCGACACAAATGCCCCCGAGGTGCTGGCCGGCGATCCTTATACCCAGGTCATCGACATCTGGTCTGCCGGCCTGGTCATCTTCGAGACAGCCGTGCACAACGCCTCCCTGTTTTCTGCCCCCAGGGATCCTGAGAGGCGCCCATGCGACTCCCAGATCGCCAGGATCATCCGCCAGGCCCAGGTGCACGTGGATGAGTTCCCTACCCACGCAGAGTCCAGACTGACCGCACACTATCGGAGCCGGGCAGCAGGAAACAATAGACCCGCCTGGACCCGGCCTGCCTGGACAAGATACTATAAGATCCACACCGATGTGGAGTATCTGATCTGTAAGGCCCTGACATTTGACGCCGCTCTGAGACCTTCCGCCGCCGAACTGCTGAGACTGCCACTGTTTCATCCAAAGTGAGAATTC |
| pUC57-ChHV-Us7o | GAATTCGGCCGACCCAACCCCCGCAGATGAAGGGACAGCAGCCGCTACAATCCTGAAACAGGCAATCGCCGGAGACAGATCACTGGTGGAGGTGGCCGAGGGCATCTCCAATCAGGCCCTGCTGAGAATGGCATGTGAGGTGCGGCAGGTGTCTGATAGACAGCCACGCTTCACCGCCACAAGCGTGGTGAGGGTGGACGTGACACCTGGCGGCAGGCTGAGATTTGTGCTGGATGGCAGCTCCGACGATGCCTACGTGGCCTCTGAGGACTATTTCAAGAGATGTGGCGGACAGCCTACCTACAGGGGCTTTGCCGTGGTGGTGCTGACAGCCAACGAAGACCACGTGCACAGCCTGGCAGTGCCCCCTCTGGTGCTGCTGCACAGACTGTCCCTGTTCAGACCAACCGACCTGAGGGATTTTGAGCTGGTGTGCCTGCTGATGTATCTGGAGAATTGTCCAAAGAGCCACGCCACACCCTCCCTGTTCGTGAAGGTGTCCGCCTGGCTGGGAGTGGTGGCAAGAAGAGCATCTCCCTTTGCCCGGGTGAGATGCCTGCTGCTGCGGTCTTGTCACTGGATCCTGAACACCCTGATGTGCATGGCCGGCATCAAGCCTTTCGACGATGGCCTGGTGCTGCCACACTGGTACATGGCCCACTATCTGCTGGCCAACAATCCACCCCCTGTGCTGAGCGCCCTGTTCTGTGCCACACCACAGTCTAGCGCCTTTCAGCTGCCAGGCCCCATCCCTCGCATGGATTGCGTGGCCTACAATCCCGCCGGCGTGATGGGCTCCTGTTGGAAGTCTAAGGACCTGCGGAGCGCCCTGGTGTATTGGTGGCTGAGCGGCAATAGGAAGCGGCGGACATCATCCCTGTTTTACCAGTTTTGTTAAGATATC |
| pUC57-ChHV-gBo769–903 | AAGCTTGAATTCTTTATGTCTAACCCCTTCGGGGCTCTGGCCGTGGGACTGCTGGTGCTGGCCGGGCTGGCTGCCGCCTTCTTTGCCTTTCGCTATGTGCTGCGGCTGCAGAGCAACCCAATGAAGGCCCTGTACCCCCTGACCACAAAGGAGCTGAAGAATCCCGACCCTCAGGGAGTCGGAGGAGAGGGAGAGGAGGGAGGAGGATTCGATGAGGCAAAGCTGGCCGAGGCCCAGGAGATGATCAGGTATATGGCCCTGGTGTCTGCCATGGAGCGCACCGAGCACAAGGCCAAGAAGAAGGGCACAAGCGCCCTGCTGAGCTCCAAGGTGACCAACATGGTGCTGCGGAAGAGAAATAAAACTCGGTATTCCCCACTGCATAATGAAGATGAGGCCGGAGATGAGGACGAACTGTGAGTCGACAAGCTT |
| pUC57-AncA-UL7o | AAGCTTGAATTCGCCGCCCCAACCCCAGACGATGAGGGAACAGCAGCAGCAATCCTGAAGCAGGCAATCGCAGGCGATAGGTCCCTGGTGGAGGTGGCCGAGGCCATCTCTCACCAGGCCCTGCTGAGGATGGCATGCGAGGTGCGCCAGGTGAGCGACAGGCAGCCAAGATTCACCGCCACATCCGTGGTGCGCGTGGACGTGACCCCAGGATGTAGGCTGCGCTTTGTGCTGGATGGCTCTCCCGACGATGCCTACGTGGCCAGCGAGGACTATTTCAAGAGGTGCTGTGGCCAGTCTACCTACAGGGGATTTGCAGTGGCCGTGCTGACAGCCAACGAGGATCACGTGCACTCCCTGGCAGTGCCACCTCTGGTGCTGCTGCACAGGCTGTCTCTGTTCCGGCCCAGAGACCTGAGAGATTTTGAGCTGGCCTGCCTGCTGATGTATCTGGAGAATTGTCCTCGGAGCCACGCCACCCCATCCACATTCGTGAAGGTGTCTGCCTGGCTGGGAGTGGTGGGCCGGAGAACCAGCCCTTTTGAGAGGGTGCGCTGCCTGCTGCTGCGGAGCTGTCACTGGATCCTAAACACACTGATGTTCATGGTGCACGTGAAGCCCTTCGACGATGAGTTTGTGCTGCCTCACTGGTACATGGCCAGATATCTGCTGGCCAACAATCCACCACCTGTGCTGTCCGCCCTGTTCTGCGCAACACCTAGCTCCGCCTTTCGGCTGCCTGGCCCAATCCCCAGATTTGATTGCGTGGCCTACAATCCAGCAGGCGTGATGGGCTCCTGTTGGGCATCTGAGGACCTGAGGGCCGCCCTGGTGTACTGGTGGCTGTCCGAGACCCCTAAGCGCCAGACATCTAGCCTGTTCTATCAGTTTTGTTGACTCGAGAAGCTT |
| pUC57-AncB-UL7o | AAGCTTGAATTCGCCGACCCAACCCCAGCAGATGAGGGAACAGCAGCAGCAATCCTGAAGCAGGCAATCGCAGGCGATCGGAGCCTGGTGGAGGTGGCAGAGGGAATCTCCAACCAGGCCCTGCTGAGAATGGCCTGCGAGGTGAGGCAGGTGTCTGACCGGCAGCCAAGATTCACCGCCACAAGCGTGGTGAGAGTGGACGTGACCCCAGGAGGCCGGCTGAGATTTGTGCTGGATGGCAGCTCCGACGATGCCTACGTGGCCAGCGAGGACTACTTCAAGCGGTGCGGAGGACAGCCAACCTACAGGGGCTTTGCCGTGGTGGTGCTGACAGCCAACGAGGATCACGTGCACAGCCTGGCAGTGCCACCTCTGGTGCTGCTGCACAGGCTGTCCCTGTTCCGCCCTACCGACCTGCGGGATTTTGAGCTGGTGTGCCTGCTGATGTATCTGGAGAATTGTCCTCGCTCTCACGCCACACCAAGCCTGTTCGTGAAGGTGTCCGCCTGGCTGGGAGTGGTGGCACGGAGAGCCTCTCCATTTGAGAGGGTGCGCTGCCTGCTGCTGCGGTCTTGTCACTGGATCCTAAATACCCTGATGTGCATGGCCGGCGTGAAGCCCTTCGACGATGAGCTGGTGCTGCCTCACTGGTACATGGCCCACTATCTGCTGGCCAACAATCCACCCCCTGTGCTGTCCGCCCTGTTCTGTGCCACACCTTCTAGCGCCTTTCAGCTGCCTGGCCCAATCCCCAGAATGGATTGCGTGGCCTACAACCCAGCCGGCGTGATGGGCAGCTGTTGGAAGTCCAAGGACCTGAGGTCCGCCCTGGTGTACTGGTGGCTGTCTGGCAATCCTAAGAGGCGCACATCCTCTCTGTTCTATCAGTTTTGCTGACTCGAGAAGCTT |
a
Restriction enzyme sites used for cloning are underlined. Open reading frame sequences are shown in black. Restriction enzyme sites not used for cloning are shown in italic font.