FIG 5.

Phenotype of ZIKV lacking the ability to generate sfRNAs in human and mosquito cells (A and B) Differential requirement of sfRNA generation for ZIKV replication in the two hosts. Luciferase expression levels of ZIKVAr-R WT and ΔSLI-II are shown as a function of time in Huh-7 and C6/36 cells. The luciferase values shown are means ± standard deviations from a representative experiment (three independent experiments were performed). (C) (Left) immunofluorescence showing ZIKV antigen positivity in C6/36 cells transfected with ZIKVAr WT and ZIKVAr ΔSLI-II. (Right) The WT and mutant virus produced high-titer stocks, as measured by focus-forming units (FFU) in C6/36 cells. (D) Representative images from immunofluorescence assays showing ZIKV antigen positivity in human A549 and Dicer-competent U4.4 mosquito cells infected with ZIKVAr WT or ZIKVAr ΔSLI-II.