FIG 3.

Thermostability of GPs from other ebolaviruses. (A) THL cleavage conditions for rVSV-SUDV GP and rVSV-BOMV GP were verified by Western blotting with anti-GP1 N-terminal peptide polyclonal rabbit sera. (B) Thermal denaturation curves for rVSVs bearing GP and GP_THL from EBOV, SUDV, and BOMV were determined with ADI-15878. Averages ± SD are shown; n = 6 from 2 independent experiments. Average A₄₅₀ range at lowest temperature tested, 0.8 to 1.1 for GP and 1.3 to 2.0 for GP_CL. (C) Calculated T_m values from the thermal denaturation curves in panel B. Averages ± SD are shown; n = 2 from 2 independent experiments.