FIG 6.

Synthesis and processing viral proteins. BS-C-1 cells were infected with 5 PFU/cell of vA8myx, vA8myxΔA26, vA8myxΔG6, or vA8myxΔA26ΔG6 or the WT virus. After 10 h and 24 h, the cells were harvested and the lysates analyzed by Western blotting using antibodies specific for E3, A17, and A3 proteins. The relative amounts of the upper (unprocessed) and lower (processed) A17 and A3 bands were determined. At 10 h, the proportions of processed A17 were 43%, 45%, 48%, 49%, and 61% and those of A3 were 22%, 20%, 24%, 29%, and 45% for vA8myx, vΔA26, vΔG6, vΔA26ΔG8, and the WT virus, respectively. At 24 h, the proportions of processed A3 were 26%, 29%, 47%, 51%, and 46% for vA8myx, vΔA26, vΔG6, vΔA26ΔG8, and the WT virus, respectively.