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. 2020 Aug 31;94(18):e00722-20. doi: 10.1128/JVI.00722-20

FIG 1.

FIG 1

HIF-1α-stabilizing drugs induce expression of lytic EBV antigens in a cell- and drug-dependent manner. (A to C) Immunoblots showing relative levels of HIF-1α, EBV IE lytic antigens (Zta and Rta), total p53, and p53 phosphorylated on Ser15 (ser15p53) present in SNU-719 (A), AGS-Akata (B), and Sal (C) cells after incubation for 24 h with the drug diluent DMSO (-), 200 μM DFO, 5 μM MLN-4924, or 5 μM FG-4592 as indicated. (D) Immunoblots showing relative levels of the indicated proteins present in BL Akata (lanes 1 to 3) and MutuI (lanes 4 to 6) cells after incubation with the drug diluent PBS (-, 24 h), DFO (200 μM, 24 h), anti-IgG (10 μg/ml, 48 h), or TGF-β (5 μg/ml, 48 h) as indicated. The last two conditions served as positive controls. GAPDH was measured as a loading control. Data are representative of those from two or more independent experiments.