FIG 2.

Reporter assays showing that FG-4592 can induce transcription from an HRE-containing promoter but not from Zp. AGS cells maintained in 24-well dishes were transfected with 200 ng of DNA/well of (i) pTATA-Luc (a minimal promoter), (ii) p3xHRE-Luc (a reporter plasmid containing three copies of the HRE from the promoter region of the PGK1 gene), or (iii) pZp-Luc. Twenty-four hours later, the drug vehicle (DMSO) or 5 μM FG-4592 was added, and incubation was continued for an additional 24 h prior to harvest and assaying for luciferase activity. Cells were incubated likewise in parallel with DFO (200 μM) and MLN-4924 (5 μM) as positive controls. Data were normalized to the activities observed with the diluent control; they represent means ± SEMs of data obtained from assays performed in triplicate on two independent occasions relative to the values obtained with pTATA-Luc.