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Genetic stability of rSA11 strains expressing fluorescent proteins. The genomes of recombinant SA11 strains NSP1-R2A-fUnaG, NSP3-P2A-fUnaG, and NSP3-P2A-fmRuby, serially passaged for 10 cycles (P1 to P10) in MA104 cells, were analyzed by gel electrophoresis. Genome segments of rSA11/wt are labeled 1 to 11. Positions of modified segment 5 (NSP1) and 7 (NSP3) dsRNAs are denoted with arrows. wt, rSA11-wt virus.
