Figure 4.

Phylogenetic tree of ompA gene sequences in bold red generated from the study with the related reference sequences obtained from NCBI GenBank. The evolutionary history was inferred using the neighbor-joining method [17]. The optimal tree with the sum of branch length = 1.67432413 is shown. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) is shown next to the branches [18]. The evolutionary distances were computed using the p-distance method [19] and are in the units of the number of base differences per site. The analysis involved 67 nucleotide sequences. Codon positions included were 1st + 2nd + 3rd + Noncoding. All positions containing gaps and missing data were eliminated. There were a total of 166 positions in the final dataset. Evolutionary analyses were conducted in MEGA7 [20]. The test sequences denoted diamond red clustered with other Rickettsia references.