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Overview of the applied workflow starting with plasma, isolation of extracellular vesicles (EV), followed by sample characterization. EVs were isolated using either high-speed centrifugation, size exclusion chromatography (SEC), or peptide affinity precipitation (PAP). EV isolates were characterized by nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), immunoblotting, and mass spectrometry (MS).
