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EV characterization by nanoparticle tracking analysis (NTA), immunoblotting, and transmission electron microscopy (TEM). (A) Differences in particle concentration measured by NTA. (B) Differences in particle mode size measured by NTA. (C) Immunoblot against CD9 (24 kDa) confirming the presence of the EV specific protein CD9 in isolates from all methods. (D) TEM images showing CD9 expression in vesicle-like particles in all three isolation methods. Error bars: Mean ± SD.
