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. 2020 Jul 25;8(8):246. doi: 10.3390/biomedicines8080246

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Difference in the co-isolation of contaminants such as serum albumin, lipoproteins, and other non-EV proteins between isolates produced by high-speed centrifugation (Cent.), size-exclusion chromatography (SEC), and peptide affinity precipitation (PAP). (A) High-speed centrifugation produced EV isolates with the highest abundance of serum albumin. (B) SEC isolates contained the highest amount of lipoproteins. (C) Immunoblotting confirms elevated levels of apolipoprotein B in isolates produced by SEC. (D) PAP isolates contained the highest amount of non-EV proteins. Error bars: Mean ± SD. Significance levels: * (p-value < 0.05) and ** (p-value < 0.01).