Table 2.
Genes | Primers Sequence ((5′-3′) | PCR Conditions | Product Sizes/(Bp) | Reference |
---|---|---|---|---|
Gyrase B (gyrB) | TCCGGCGGTCTGCACGGCGT TTGTCCGGGTTGTACTCGTC |
• Initial denaturation 94 °C/5 min. • 30 cycles of denaturation at 94 °C/30 s • Annealing at 59 °C/30 s • Extension at 72 °C/1 min |
1100 | [20] |
Aerolysin (aer) | AACCGAACTCTCCAT CGCCTTGTCCTTGTA |
• Initial denaturation step at 94 °C/5 min • 30 cycles with denaturation at 94 °C/30 s • Annealing at 54 °C/30 s • Extension at 72 °C/1 min |
301 | [21] |
Elastase (ahp) | ACACGGTCAAGGAGATCAAC CGCTGGTGTTGGCCAGCAGG |
• Initial denaturation: 94 °C/4 min • 35 cycles of denaturation at 94 °C for 30 s • Annealing at 55.5 °C for 30 s • Extension step at 72 °C for 30 s |
540 | [27] |
Hemolysin (hyl) | CACAGCCAATATGTCGGTGAAG GTCACCTTCTCGCTCAGGC |
• Same amplification conditions for elastase gene except for annealing at 60.6 °C for 30 s | 326 | [22] |
Lipase (lip) | ATCTTCTCCGACTGGTTCGG CCGTGCCAGGACTGGGTCTT |
• Same amplification conditions for elastase gene except for annealing at 58.2 °C for 30 s | 383–389 | [27] |