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. 2020 Aug 16;10(8):1432. doi: 10.3390/ani10081432

Table 2.

The primer sets, PCR conditions, and size of the amplified sequence of Aeromonas isolates.

Genes Primers Sequence ((5′-3′) PCR Conditions Product Sizes/(Bp) Reference
Gyrase B (gyrB) TCCGGCGGTCTGCACGGCGT
TTGTCCGGGTTGTACTCGTC
• Initial denaturation 94 °C/5 min.
• 30 cycles of denaturation at 94 °C/30 s
• Annealing at 59 °C/30 s
• Extension at 72 °C/1 min
1100 [20]
Aerolysin (aer) AACCGAACTCTCCAT
CGCCTTGTCCTTGTA
• Initial denaturation step at 94 °C/5 min
• 30 cycles with denaturation at 94 °C/30 s
• Annealing at 54 °C/30 s
• Extension at 72 °C/1 min
301 [21]
Elastase (ahp) ACACGGTCAAGGAGATCAAC
CGCTGGTGTTGGCCAGCAGG
• Initial denaturation: 94 °C/4 min
• 35 cycles of denaturation at 94 °C for 30 s
• Annealing at 55.5 °C for 30 s
• Extension step at 72 °C for 30 s
540 [27]
Hemolysin (hyl) CACAGCCAATATGTCGGTGAAG
GTCACCTTCTCGCTCAGGC
• Same amplification conditions for elastase gene except for annealing at 60.6 °C for 30 s 326 [22]
Lipase (lip) ATCTTCTCCGACTGGTTCGG
CCGTGCCAGGACTGGGTCTT
• Same amplification conditions for elastase gene except for annealing at 58.2 °C for 30 s 383–389 [27]