Figure 9.

circRNA8220 activated the signaling pathways of Ras/MEK/ERK and PI3K/AKT/mTOR in GMEC. (a,b) Western blot analysis was performed to detect the protein expression of Ras, p-MEK, MEK, p-ERK44/42, ERK44/42, p-PI3K, PI3K, p-AKT, AKT, p-mTOR, mTOR, p-S6K, S6K and β-actin in GMEC after transfection with PCD5-circRNA8220 or si-circRNA8220. Protein levels were measured by WB densitometry was normalised to the β-actin density from the same lane. Data were expressed as the means ± SEM. ** p < 0.01, * p < 0.05.