Table 3.
Common macrophagic and epithelial infection models for nontuberculous mycobacteria drug testing.
| Cell Type | PMA | Bacteria | MOI | Reference |
|---|---|---|---|---|
| Human THP-1 | 60–500 ng/mL for 24–48 h |
MAC | 10 for 1 h | [26,27] |
| Mycobacteroides abscessus | 1 or 10 for 1–3 h | [26,27,29] | ||
| Human U937 | 1 µg/mL for 24 h | MAC | 5 for 4 h | [30] |
| Human A549 | NA | MAC | 10 for 2 h | [38] |
| NA | MAC | 10 or 20 for 2–3 h | [36,37] | |
| Murine J774 | NA | Mycobacterium smegmatis | 1 for 3 h | [43] |
| NA | MAC | 5 for 4 h or 20 for 3 h | [40,42,74] | |
| NA | Mycobacteroides abscessus | 5 for 4 h | [40] | |
| Murine RAW264.7 | NA | Mycobacteroides abscessus | 10 for 2 h | [41] |
| BMDMs | NA | MAC | 1 for 4–5 h | [50] |
| NA | Mycobacteroides abscessus | 3 for 4 h | [49] |
Abbreviations: BMDMs, murine primary bone marrow-derived macrophages; MAC, Mycobacterium avium complex; MOI, multiplicity of infection; PMA, phorbol 12-myristate 13-acetate; NA, not applicable.