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. 2020 Jul 28;10(8):1283. doi: 10.3390/ani10081283

Figure 2.

Figure 2

mRNA expression levels of genes encoding intestinal pro-inflammatory mediators in ileal Peyer’s patches of postweaning piglets supplemented with mannan oligosaccharides. Data shown as LSMeans ± SEM. Values are expressed as relative arbitrary unit (RAU). CON (n = 10): animals receiving the basal diet with no MOS supplementation; TRT (n = 10): animals receiving the basal diet with 0.2% MOS supplementation; PCR reactions were normalised to the Ct values of the reference genes β-actin and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) of S. scrofa, and differential expressions were determined using the comparative Ct method; LSMeans: least-square means; SEM: standard error of the mean; * significant at p < 0.05; ** significant at p < 0.01.