Table 4. Unaligned reads identified by ROP in the simulated dataset.

Dataset	Unaligned reads identified	Low Quality Reads	Low Complexity Reads	rRNA reads	Lost Reads	Repeat reads	NCL Reads	Immune Reads	Microbial Reads
T1	31,469	1	188	251	30,398	502	9	120	1
T2	27,328	0	306	148	23,508	1,690	28	1,639	9
T3	58,544	3	2,469	13	3,085	7,123	132	45,696	24

The result shown is an average from 3 samples.