Table 8. Alignment statistics for simulated datasets before and after Scavenger recovery using optimised parameters for Subread.
Dataset | Source execution | Scavenger pipeline | Unaligned
reads recovered |
% recovered
reads correct |
% recovered
reads incorrect |
||||
---|---|---|---|---|---|---|---|---|---|
Aligned
correctly |
Aligned
incorrectly |
Unaligned | Aligned
correctly |
Aligned
incorrectly |
Unaligned | ||||
T1 | 9,416,480 | 262,926 | 320,594 | 9,419,057 | 264,906 | 316,037 | 4,557 | 56.5% | 43.5% |
T2 | 9,283,792 | 397,323 | 318,885 | 9,287,022 | 398,775 | 314,203 | 4,682 | 69.0% | 31.0% |
T3 | 7,111,603 | 2,251,068 | 637,330 | 7,122,864 | 2,251,625 | 625,512 | 11,818 | 95.3% | 4.7% |
The result shown is an average from 3 samples.