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. 2020 Jul 27;10(8):124. doi: 10.3390/life10080124

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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SDS-PAGE analysis of PICUP Samples. (A) Samples from PICUP reactions with ^NAcαSyn variants supplemented with no-metal, Fe^II_, or Fe^III separated on 10% SDS-PAGE gels and stained with Coomassie blue R-250. Ladder is located to the left of the gels. (B) Schematic representation of intramolecular and intermolecular dityrosine crosslinks. (C) Concentrated samples of PICUP reactions performed with ^NAcY125F (yellow) and ^NAcY133F (green) separated on 10% SDS-PAGE gels stained with Coomassie blue R-250.