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Primer sequences used for real-time PCR of HSP70 gene in the liver.
| Gene | Primer Sequence (5′–3′) | PCR Product (bp) | Melting Temperature (°C) |
|---|---|---|---|
| HSP70 | F: GCTTATGGTGCCGCTGTG R: TGGTGGTGTTACGCTTGATG |
151 | 55.7 |
| 18SrRNA | F: GACACGGACAGGATTGACAG R: CCAGAGTCTCGTTCGTTATCG |
120 | 55.6 |
