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. 2020 Aug 4;9(8):478. doi: 10.3390/antibiotics9080478

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Lactobacillus plantarum 108 supernatant (Sup) inhibited S. mutans (Sm) biofilm formation and demonstrated activity against preformed biofilms. Lp108 supernatant was introduced at two time points; 0 h for the preventive assay and at 12 h into a preformed biofilm for the therapeutic assay. Biofilms were quantified using (A) XTT reduction assay, (B) Crystal Violet assay and (C) Colony forming unit counting (CFU). Data are presented as mean ± SD and statistical significance indicated by the asterisk (*) was evaluated with respect to the control group without the Lp108 supernatant treatment (p < 0.05).

Lactobacillus plantarum 108 supernatant (Sup) inhibited S. mutans (Sm) biofilm formation and demonstrated activity against preformed biofilms. Lp108 supernatant was introduced at two time points; 0 h for the preventive assay and at 12 h into a preformed biofilm for the therapeutic assay. Biofilms were quantified using (A) XTT reduction assay, (B) Crystal Violet assay and (C) Colony forming unit counting (CFU). Data are presented as mean ± SD and statistical significance indicated by the asterisk (*) was evaluated with respect to the control group without the Lp108 supernatant treatment (p < 0.05).