Figure 6.

Lactobacillus plantarum 108 supernatant down-regulated the gtf gene expression in Streptococcus mutans single and mixed-species biofilms. Quantitative PCR was performed to evaluate the expression of gtfB, gtfC, and gtfD genes in (A) single and (B) mixed species biofilms treated with Lp108 supernatant. Target genes in the sample were normalized with the S. mutans housekeeping gene 16sRNA. The graphs show the relative expression fold change of treated group with respect to the control group. Data represent the mean ± SD of three independent experiments and statistical significance (*) with respect to the control group (p < 0.05).