Table 1.
Histopathological changes in tissues investigated in domestic pigs and wild boar.
| Organ | Lesion (Scored with Normal (0), Mild (1), Moderate (2), Severe (3) | ||||||
|---|---|---|---|---|---|---|---|
| Spleen | Apoptosis of Lymphoid Cells (Syn. Lymphocytolysis) | Apoptosis/Necrosis in the Red Pulp | Hyperplasia/Hypertrophy of Myelomonocytic Cells (i.e., Dendritic Cells, Monocytes, Macrophages) | ||||
| Liver with gall bladder | Congestion/hemorrhage | Sinusoidal inflammatory infiltrates | Degeneration/necrosis and loss of Kupffer cells | Hyperplasia of bile duct epithelium | |||
| Lymph nodes | Congestion/hemorrhage | Apoptosis of lymphoid cells | Apoptosis/necrosis, perifollicular cortex and paracortex | Presence of tingible body macrophages | Hyperplasia of lymphocytes | ||
| Palatine tonsil | Congestion/hemorrhage | Apoptosis of lymphoid cells | Apoptosis/necrosis, perifollicular cells | Crypt abscessation | |||
| Lung | Congestion/hemorrhage | Edema | Inflammation | Hyperplasia/hypertrophy of alveolar and interstitial monocytes/macrophages | |||
| Kidney | Congestion/hemorrhage | Inflammatory infiltrates | |||||
| Brain | Inflammatory infiltrates | Neuronal degeneration | Gliosis | ||||
| Bone marrow | Necrosis | ||||||
Immunohistochemically stained sections were scored for viral antigen distribution and scored on a 0–3 scale. The most affected area per sample sections was scored with no antigen (0), < 1–3 positive cells (1), 4–15 cells (2), and >16 cells (3) per high (40×) power field. Cells showing fine granular cytoplasmic labelling were considered positive; chromogen aggregations without cellular association were not counted.