Skip to main content
. 2020 Jul 31;8(8):258. doi: 10.3390/biomedicines8080258

Figure 4.

Figure 4

Combination of fasudil (5 mg) and menthol (10 mg) attenuates apoptosis and facilitates M2 polarization following spinal cord injury. (A) Representative immunofluorescence images for Caspase-3 and (B) quantitative fluorescence intensity of caspase-3. 4′,6-diamidino-2-phenylindole (DAPI) was used as an internal control (Blue). (C) Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay showing apoptotic bodies following SCI in the representative spinal cord epicenter and (D) quantitative result of the TUNEL assay. (E) Representative immunofluorescence images of spinal cord epicenter for Arginase-1 (Arg-1) and (F) quantitative fluorescence intensity of Arginase-1. Scale bar: 20 µm, florescence quantification data are expressed in mean ± SEM, n = 3/group. F10, fasudil. 10 mg/kg i.p. M10, menthol 10 mg/kg i.p., F + M, Fasudil5 mg/kg + Menthol 10 mg/kg i.p., ### p < 0.001 (sham vs. injury), + p < 0.05, ++ p < 0.01 (fasudil vs. injury), $ p < 0.05 (menthol vs. injury), ^^ p < 0.05 (combination vs. fasudil 10 mg), and ** p < 0.01, *** p < 0.01 (combination vs. menthol 10 mg).