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. 2020 Jul 31;8(8):258. doi: 10.3390/biomedicines8080258

Figure 6.

Figure 6

Combination of fasudil (5 mg) and menthol (10 mg) prevents fibrotic scarring and plays a significant role in angiogenesis and axonal regeneration following SCI. (A) Representative immunofluorescence images for transforming growth factor-β1 (TGF-β1), growth cone, and angiopoietin-1 (ANGPT-1). The experimental procedure is explained in the Materials and Methods section. (B) Quantitative fluorescence intensity of TGF-β1, (C) quantitative fluorescence intensity of growth cone, and (D) quantitative fluorescence intensity of ANGPT-1 (mean ± SEM, n = 2–3/group), (scale bar = 20 µm). Tissues were collected at 28 days after SCI. ### p < 0.001 (sham vs. injury), ++ p < 0.01, +++ p < 0.001 (fasudil vs. injury) ^^ p < 0.01, ^^^ p < 0.001 (combination vs. fasudil 10 mg), and * p < 0.05, *** p < 0.001 (combination vs. menthol 10 mg).