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. 2020 Aug 14;10(8):1417. doi: 10.3390/ani10081417

Figure 6.

Figure 6

Dual-luciferase reporter assay validates the interaction between hsa-miR-212-5p and target genes. (A) has-miR-212-5p and its target genes (TargetScan and Miranda were used for the prediction. Target genes with context score percentile >80 and max energy <−18 were shown). (B) Interspecies conservation of miR-212-5p. The potential binding sites on FGFRL1 and IGF2BP1 are shown. The mutated sequence is shown in red. hsa, mmu, bta, cfa, gga, and apl represent Homo sapiens, Mus musculus, Bos taurus, Canis lupus familiaris, Gallus gallus, and Anas platyrhynchos. (C,D) Relative luminescence was measured and calculated after co-transfecting a wild type or mutant sequence of FGFRL1 and IGF2BP1 with miR-212-5p mimic (or mimic NC) in DF-1. In all panels, values are presented as mean ± SEM. * p < 0.05.