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Binding of RBP-reporters to inactivated cells of Y. pestis. Cells of Y. pestis EV76 grown for 7 h at 28 °C were inactivated as indicated, washed with PBS buffer, incubated with fusion proteins mCherry-RBP (phage L-413C; red signals, upper panels) or mCherry-RBP (phage ΦA1122; red signals lower panels) and subjected to fluorescence microscopy (recorded in merged light and fluorescence channels). Untreated cells served as positive controls (scale bar: 5 µm).
