Table 2.

Results of recovery experiment 2 after spiking and washing 300 g of lettuce with different numbers of sodium hypochlorite resistant (SHR) eggs of Echinococcus multilocularis and sporulated oocysts of Toxoplasma gondii. PCR results show the proportion of positives out of five or three replicates.

Environmental Stages Used for Spiking
Echinococcus multilocularis Eggs Containing SHR Oncospheres			Toxoplasma gondii Oocysts
# Eggs (Volume of Spiking Suspension)	PCR 1 1st half	PCR 2nd half	# Oocysts	PCR 2
120 (779 µL)	5/5		86	5/5
40 (260 µL)	5/5		18	4/5
20 (130 µL)	5/5		0	0/3
10 (65 µL)	5/5	5/5
2 3 (20 µL)	5/5	1/5
0 (none)	0/3	0/3
0 (779 µL 4)	0/3	-
0 (130 µL 4)	0/3	-

¹ Trachsel et al. [26], ² Burg et al. [27], ³ isolated by microscopy without SHR testing, ⁴ egg-free supernatants of the spiking suspension.