Table A1.

Primers and probes used for RT-PCR.

Gene Symbol RefSeq Accession Number	The Encoded Protein and Its Function	Primer 5′–3′ (Forward, Reverse, Probe)	Amplicon Length and Position on mRNA	Reference
Gapdh1 NM_017008	Glyceraldehyde-3-phosphate dehydrogenase Glycolytic enzyme	TGCACCACCAACTGCTTAG GGATGCAGGGATGATGTTC ATCACGCCACAGCTTTCCAGAGGG *	177 bp (523–699)	[49]
B2m1 NM_012512	Beta-2-microglobulin Assembly and surface expression of MHC class I molecules	TGCCATTCAGAAAACTCCCC GAGGAAGTTGGGCTTCCCATT ATTCAAGTGTACTCTCGCCATCCACCG&	73 bp (77–149)	[50]
Actb1 NM_031144	Beta-actin Cytoskeletal protein	TGTCACCAACTGGGACGATA GGGGTGTTGAAGGTCTCAAA CGTGTGGCCCCTGAGGAGCAC #	165 bp (303–467)	[51] (forward and reverse primers) [30] (hydrolysis probe)
Ppia2 NM_017101	Peptidyl prolyl isomerase A, cyclophilin A Protein folding	AGGATTCATGTGCCAGGGTG CTCAGTCTTGGCAGTGCAGA CACGCCATAATGGCACTGGTGGCA &	187 bp (216–402)	[33]
Rpl13a2 NM_173340	Ribosomal protein L13A 60S ribosomal subunit protein	GGATCCCTCCACCCTATGACA CTGGTACTTCCACCCGACCTC CTGCCCTCAAGGTTGTGCGGCT #	131 bp (334–464)	[2] (forward and reverse primers) [30] (hydrolysis probe)
Sdha3 NM_130428	Succinate dehydrogenase complex, subunit a, flavoprotein Component of electron transport chain	AGACGTTTGACAGGGGAATG TCATCAATCCGCACCTTGTA ACCTGGTGGAGACGCTGGAGCT *	160 bp (1666–1825)	[52] (forward and reverse primers) [30] (hydrolysis probe)
Ywhaz3 NM_013011	Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide Cell signaling	GATGAAGCCATTGCTGAACTTG GTCTCCTTGGGTATCCGATGTC TGAAGAGTCGTACAAAGACAGCACGC &	117 bp (650–766)	[14] (forward and reverse primers) [30] (hydrolysis probe)
Pgk13 NM_053291	Phosphoglycerate kinase 1 Glycolytic enzyme	ATGCAAAGACTGGCCAAGCTAC AGCCACAGCCTCAGCATATTTC TGCTGGCTGGATGGGCTTGGA *	104 bp (888–991)	[14] (forward and reverse primers) [30] (hydrolysis probe)
Hprt3 NM_012583	Hypoxanthine phosphoribosyl transferase 1 Purine synthesis	TCCTCAGACCGCTTTTCCCGC TCATCATCACTAATCACGACGCTGG CCGACCGGTTCTGTCATGTCGACCCT #	80 bp (28–107)	[53] (forward and reverse primers) [30] (hydrolysis probe)

* Labeled with R6G fluorescent dye (5′) and BHQ2 quencher (3′). # Labeled with FAM fluorescent dye (5′) and BHQ1 quencher. & Labeled with Rox fluorescent dye (5′) and BHQ1 quencher (3′). ^{1, 2, 3} —assays with the same indeces were run in a single multiplex reaction.